Analysis of Trp aminoacyl synthetase.

Analysis performed on 2024-07-10 11:40:01

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 47200 503 46697 17863
Archaea 3403 83 3320 1423
Vertebrata 1311 217 1094 497
Mammals 536 75 461 126

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
471 26 315

Domains

Domains
Current Study 1, 52
Guo et al (2010) 8, 64

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 420 51 471 8.235294
Number of Variants 296 45 341 6.577778
Number of Pathogenic Variants 22 4 26 5.500000
Number of Benign/Unknown Variants 274 41 315 6.682927
Fisher exact test 0.761847

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 414 57 471 7.263158
Number of Variants 288 53 341 5.433962
Number of Pathogenic Variants 21 5 26 4.200000
Number of Benign/Unknown Variants 267 48 315 5.562500
Fisher exact test 0.575133

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 420 51 0.89 (0.01) 0.78 (0.02) 9.503e-01 7.752e-01 15221.50 4.44e-07
Guo et al (2010) 414 57 0.89 (0.01) 0.81 (0.02) 9.502e-01 8.050e-01 15775.50 1.84e-05

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains